Materials science of dna download

PDF - 1. Fundamental Concepts cont. PDF - 2. The Hydrogen Atom cont. The Periodic Table cont. Fundamental Equations cont. Quantum Oscillation PDF. Electrochemical Equilibria PDF. PDF - 3. Symmetry Operations PDF. PDF - 9. Structure of Solids PDF - 1. Single-Component Phase Diagrams cont. X-ray Diffraction PDF - 1. Binary Phase Diagrams cont. Discovery of the structure of DNA Opens a modal. DNA replication. When you make a copy of something, how perfect does the copy need to be?

If that something is your DNA, the answer is, pretty perfect! Errors during DNA copying can lead to cancer and other health conditions.

Learn how cells use a group of different enzymes to accurately copy their DNA, and how they detect and repair copying errors and DNA damage. Leading and lagging strands in DNA replication Opens a modal. Speed and precision of DNA replication Opens a modal. Molecular mechanism of DNA replication Opens a modal. DNA proofreading and repair Opens a modal. Telomeres and telomerase Opens a modal. The sample is injected in the labcard and, after the elution of the DNA, it is transferred to the PCR chamber, where the DNA is amplified and transferred to the detection chamber.

In this chamber an electrochemical sensor is integrated. In this work we have developed an electrochemical detection system EDS , with the aim to simplify and reduce the cost of a Lab on a Chip LOC device [1] in the case of qualitative DNA detection measurements. PublishedbyElsevier Ltd.

Published by Elsevier Ltd. LOC devices have achieved a wide popularity as they have opened the possibilities of portable analysis in contrast to complex laboratory assays. The addition of an electrochemical detection system to a LOC device can provide significant advantages.

Electrochemistry detection can provide high sensitivity in addition to low cost, fast response and small dimensions which are key factors for a portable device. To fulfill these requirements, the main objective of this work is to develop a low cost system with integrated electrodes and electronics [2], which improves the signal to noise ratio of the electrochemical sensor.

The electrochemical sensors are integrated in the electrochemical detection chamber of the labcard. For that, gold and platinum electrodes are deposited by sputtering on COC cycloolefin copolymer achieving a total integration of the electrodes in the microfluidic cartridge, as it is shown in Fig 1.

Fig 1. Electrochemical sensor, with sputtered gold working electrode and sputtered platinum counter and reference electrodes integrated in the microfluidic cartridge. In Fig 2, the block diagram of the fabricated electrochemical module is shown. This module consists of three main parts: impedance matching, current measurement and error signal amplification,.

The current measurement module allows to perform a configuration with single or double power supply mode. If the currents that are going to be measured in the application are higher than nA, the single power supply mode is used for cost reduction. Since the microfluidic cartridge exhibits a high degree of integration, the read-out and the processing unit for the system have to combine the circuitry necessary for the cartridge control and the hardware which is required for the particular sensor.

Therefore, in order to integrate all the electronics of the system, the control electronics of the processing unit of the LOC is used for the cartridge control and also for the hardware which is required for the electrochemical sensor. The fabricated electronic card is shown in Fig 3. Finally different tests are made to see the repeatability of the measurement. Fig 4 b shows the mean values and the obtained highest and lowest currents in measurements carried out in different sensors.

Fig 4. The addition of the described electrochemical detection system to the LOC device, has enabled the fabrication of a low cost, fast response and small dimension device. Although the EDS is not able to make quantitative measurement of DNA, it is able to make qualitative measurements with a detection limit that is enough for the desired application, i. Ruano-Lopez, F. Laouenan, M.